Hotspot

ndhA

SSC · 114131-116279 · 2149 bp

Back to Nordenstamia

Pi 0.00248

MarkerSeek score 48.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2149
variable_sites: 8
pi: 0.00248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTCCCCCGGGGTTTATTAAT	TGTTTGAGACAAGTCGTGAA	50.0	40.0	56.9	55.2	2303-2304 bp	1.0	49.4
ndhA_p2	GTCCCCCGGGGTTTATTAAT	TTGTTTGAGACAAGTCGTGA	50.0	40.0	56.9	55.2	2304-2305 bp	1.0	49.4
ndhA_p3	GTCCCCCGGGGTTTATTAAT	TTGTTTGAGACAAGTCGTGAA	50.0	38.1	56.9	56.0	2304-2305 bp	1.0	47.8
ndhA_p4	GTCCCCCGGGGTTTATTAATT	TGTTTGAGACAAGTCGTGAA	47.6	40.0	57.7	55.2	2303-2304 bp	1.0	47.3
ndhA_p5	GTCCCCCGGGGTTTATTAATT	TTGTTTGAGACAAGTCGTGA	47.6	40.0	57.7	55.2	2304-2305 bp	1.0	47.3