Hotspot

trnS-GCT-trnC-GCA

LSC · 8709-9453 · 745 bp

Back to Nordenstamia

Pi 0.017

MarkerSeek score 69.30

Primer available yes

Label trnS-GCT-trnC-GCA

Feature Metadata

label_name: trnS-GCT-trnC-GCA
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 14
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	804-896 bp	1.0	83.4
trnS-GCT-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	786-878 bp	1.0	81.3
trnS-GCT-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	740-832 bp	1.0	79.7
trnS-GCT-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	805-897 bp	1.0	79.2
trnS-GCT-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	722-814 bp	1.0	77.5