Hotspot

petD

LSC · 77849-79121 · 1273 bp

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Pi 0.00236
MarkerSeek score 60.10
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1273
variable_sites
3
pi
0.00236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 AGTTGAAGTGGATTCTCGAAGA ACACGATCCGTATTTTCGCA 40.9 45.0 57.1 58.0 1394 bp 1.0 57.2
petD_p2 AGTTGAAGTGGATTCTCGAAGA CACACGATCCGTATTTTCGC 40.9 50.0 57.1 57.7 1395 bp 1.0 54.4
petD_p3 AGTTGAAGTGGATTCTCGAAGA GCACACGATCCGTATTTTCG 40.9 50.0 57.1 57.7 1396 bp 1.0 54.4
petD_p4 GTTGAAGTGGATTCTCGAAGA ACACGATCCGTATTTTCGCA 42.9 45.0 55.7 58.0 1393 bp 1.0 53.4
petD_p5 AGTTGAAGTGGATTCTCGAAG ACACGATCCGTATTTTCGCA 42.9 45.0 55.6 58.0 1394 bp 1.0 53.3