Hotspot

petB

LSC · 76749-77631 · 883 bp

Back to Nolina

Pi 0.00454
MarkerSeek score 61.30
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
883
variable_sites
4
pi
0.00454
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 AAGAGTCGATCCGCATTACG ATTGGTCCTCCCCGATATGA 50.0 50.0 57.9 57.6 1065-1066 bp 1.0 76.0
petB_p2 AAGAGTCGATCCGCATTACG TTGGTCCTCCCCGATATGAT 50.0 50.0 57.9 57.6 1064-1065 bp 1.0 76.0
petB_p3 AAAACAAGAGTCGATCCGCA ATTGGTCCTCCCCGATATGA 45.0 50.0 57.8 57.6 1070-1071 bp 1.0 75.7
petB_p4 AAAACAAGAGTCGATCCGCA TTGGTCCTCCCCGATATGAT 45.0 50.0 57.8 57.6 1069-1070 bp 1.0 75.7
petB_p5 GAGGCCTGTAACGATCAACA TTGGTCCTCCCCGATATGAT 50.0 50.0 57.6 57.6 992-993 bp 1.0 74.0