Hotspot

ndhE

SSC · 119671-119976 · 306 bp

Pi 0.00327

MarkerSeek score 46.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGACAAGCGGATTCACATCT	TGGACTGTTGGAGATGGAGT	50.0	50.0	58.0	58.0	1319 bp	1.0	79.9
ndhE_p2	ACATCCGTCCCAAGGTATCA	TGGACTGTTGGAGATGGAGT	50.0	50.0	58.1	58.0	1245 bp	1.0	79.1
ndhE_p3	GAGTACGGGTTTTTGTCGGT	TGGACTGTTGGAGATGGAGT	50.0	50.0	58.1	58.0	1451 bp	1.0	79.1
ndhE_p4	CCAATACATCTGTGGGGCAA	TGGACTGTTGGAGATGGAGT	50.0	50.0	57.9	58.0	1222 bp	1.0	79.0
ndhE_p5	GGACAAGCGGATTCACATCT	TATTTTGTAGCTGCCGCACA	50.0	45.0	58.0	58.2	1430 bp	1.0	78.6