Hotspot

ccsA

SSC · 116203-117174 · 972 bp

Back to Nolina

Pi 0.00103
MarkerSeek score 50.50
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
1
pi
0.00103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GAGTGGCGGCATACTGTAAA AACCTGCACAAAGTTTCCGT 50.0 45.0 58.0 58.5 1124 bp 1.0 75.7
ccsA_p2 GAGTGGCGGCATACTGTAAA ACCTGCACAAAGTTTCCGTT 50.0 45.0 58.0 58.5 1123 bp 1.0 75.7
ccsA_p3 GAGTGGCGGCATACTGTAAA AAACCTGCACAAAGTTTCCG 50.0 45.0 58.0 57.4 1125 bp 1.0 75.3
ccsA_p4 GAGTGGCGGCATACTGTAAA TGCACAAAGTTTCCGTTACG 50.0 45.0 58.0 57.3 1120 bp 1.0 74.0
ccsA_p5 GAGTGGCGGCATACTGTAAA GCACAAAGTTTCCGTTACGT 50.0 45.0 58.0 57.3 1119 bp 1.0 74.0