Hotspot

atpF

LSC · 12434-13820 · 1387 bp

Back to Nolina

Pi 0.00216
MarkerSeek score 52.00
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1387
variable_sites
3
pi
0.00216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACTAATTTCGTCAGCTCGAA AAAAAGGGCGAGTCAAGTGA 40.0 45.0 54.9 57.7 1567 bp 1.0 52.2
atpF_p2 ACTAATTTCGTCAGCTCGAA GGGCGAGTCAAGTGATACAA 40.0 50.0 54.9 57.6 1562 bp 1.0 51.9
atpF_p3 ACTAATTTCGTCAGCTCGAA AGGGCGAGTCAAGTGATACA 40.0 50.0 54.9 58.4 1563 bp 1.0 51.4
atpF_p4 ACTAATTTCGTCAGCTCGAA AAAAGGGCGAGTCAAGTGAT 40.0 45.0 54.9 57.4 1566 bp 1.0 50.4
atpF_p5 ACTAATTTCGTCAGCTCGAA AAGGGCGAGTCAAGTGATAC 40.0 50.0 54.9 57.3 1564 bp 1.0 49.6