Hotspot

rps3-rpl22

LSC · 84834-84901 · 68 bp

Back to Nolina

Pi 0.0882
MarkerSeek score 86.00
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
68
variable_sites
6
pi
0.0882
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 GTTGTGCGAACCAAAAGGAA TTAAGACCCAGGGCTAGAGG 45.0 55.0 57.4 57.8 234 bp 1.0 76.1
rps3-rpl22_p2 TGTGCGAACCAAAAGGAATG TTAAGACCCAGGGCTAGAGG 45.0 55.0 57.2 57.8 232 bp 1.0 74.3
rps3-rpl22_p3 CCAAGTCTGAAACCAAGTGGA TTAAGACCCAGGGCTAGAGG 47.6 55.0 58.1 57.8 196 bp 1.0 72.5
rps3-rpl22_p4 GTGCGAACCAAAAGGAATGA TTAAGACCCAGGGCTAGAGG 45.0 55.0 56.9 57.8 231 bp 1.0 72.0
rps3-rpl22_p5 GTTGTGCGAACCAAAAGGAA TAAGACCCAGGGCTAGAGGA 45.0 55.0 57.4 58.7 233 bp 1.0 72.0