Hotspot

ycf4-cemA

LSC · 61273-62109 · 837 bp

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Pi 0.00239
MarkerSeek score 60.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
837
variable_sites
2
pi
0.00239
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGACTCCGCGAGAAATTGAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.0 57.8 987 bp 1.0 78.9
ycf4-cemA_p2 TGAGAATTTGACTCCGCGAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.0 57.8 995 bp 1.0 78.9
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA ATGAGAGAGAGACCCACCAG 45.0 55.0 57.9 57.8 939 bp 1.0 78.3
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.1 57.8 941 bp 1.0 78.0
ycf4-cemA_p5 AAAAAGCTGCTGAATTGGCC ATGAGAGAGAGACCCACCAG 45.0 55.0 57.8 57.8 966 bp 1.0 77.5