Hotspot

psaI-ycf4

LSC · 60370-60717 · 348 bp

Back to Nolina

Pi 0.0115
MarkerSeek score 60.10
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
348
variable_sites
4
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCGATTGCAATGGCTTCTTT ACTTGCTTTTCGAGACCCTG 45.0 50.0 58.0 58.1 462 bp 1.0 79.8
psaI-ycf4_p2 GCGATTGCAATGGCTTCTTT CTTGCTTTTCGAGACCCTGT 45.0 50.0 58.0 58.1 461 bp 1.0 79.8
psaI-ycf4_p3 GTGGGCCTAGTATTTCCTGC ACTTGCTTTTCGAGACCCTG 55.0 50.0 58.0 58.1 480 bp 1.0 79.5
psaI-ycf4_p4 GTGGGCCTAGTATTTCCTGC CTTGCTTTTCGAGACCCTGT 55.0 50.0 58.0 58.1 479 bp 1.0 79.5
psaI-ycf4_p5 GCGATTGCAATGGCTTCTTT AAAAAGGATACAGGCCCAGC 45.0 50.0 58.0 58.1 489 bp 1.0 79.5