Hotspot

trnC-GCA-petN

LSC · 28639-29572 · 934 bp

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Pi 0.00214
MarkerSeek score 60.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
934
variable_sites
2
pi
0.00214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.5 58.0 1015 bp 1.0 75.9
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTA AGCAATTAGTAGTGCCCAGC 50.0 50.0 57.5 58.0 1092 bp 1.0 75.6
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTA AGTCCACTCCTTCCCCATAC 50.0 55.0 57.5 58.1 1067 bp 1.0 75.0
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTA GTCCACTCCTTCCCCATACT 50.0 55.0 57.5 58.1 1066 bp 1.0 75.0
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTA GAGTCCACTCCTTCCCCATA 50.0 55.0 57.5 57.8 1068 bp 1.0 74.5