Hotspot

trnS-GCU-trnG-GCC

LSC · 8459-9731 · 1273 bp

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Pi 0.00157
MarkerSeek score 59.70
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1273
variable_sites
2
pi
0.00157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1407 bp 1.0 69.8
trnS-GCU-trnG-GCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 1411 bp 1.0 69.8
trnS-GCU-trnG-GCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1406 bp 1.0 69.7
trnS-GCU-trnG-GCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 1407 bp 1.0 69.7
trnS-GCU-trnG-GCC_p5 GATCAGAGGGAACGGAGAGA CGAACGAATCGCACTTTTACC 55.0 47.6 57.9 58.2 1410 bp 1.0 69.7