Hotspot

rps18

LSC · 65959-66264 · 306 bp

Back to Noccaea

Pi 0.00327

MarkerSeek score 40.60

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	GGTTTCATGGCCAAGGGTAA	TTTAGTTTCGGCTCATCGGG	50.0	50.0	58.1	58.0	1238-1241 bp	1.0	80.6
rps18_p2	AAGGAGGTTTCATGGCCAAG	TTTAGTTTCGGCTCATCGGG	50.0	50.0	58.1	58.0	1243-1246 bp	1.0	80.6
rps18_p3	TCCAAAGGAGGTTTCATGGC	TTTAGTTTCGGCTCATCGGG	50.0	50.0	58.1	58.0	1247-1250 bp	1.0	80.5
rps18_p4	GGTTTCATGGCCAAGGGTAA	ACGCGGATATATAGCTCGGA	50.0	50.0	58.1	58.0	1349-1352 bp	1.0	80.4
rps18_p5	AAGGAGGTTTCATGGCCAAG	ACGCGGATATATAGCTCGGA	50.0	50.0	58.1	58.0	1354-1357 bp	1.0	80.3