Hotspot

ccsA

SSC · 112963-113949 · 987 bp

Back to Noccaea

Pi 0.0152

MarkerSeek score 59.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 15
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTTTTGAACAAGTTGCCGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1781-1783 bp	1.0	80.0
ccsA_p2	AAGTTGCCGCTATGGTGAAA	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1772-1774 bp	1.0	79.8
ccsA_p3	TAGACACGCTGCTCTTAGGA	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	1748-1750 bp	1.0	79.6
ccsA_p4	AGTTGCCGCTATGGTGAAAT	TTATCGATGGCTTCCCTTGC	45.0	50.0	57.9	58.0	1771-1773 bp	1.0	79.6
ccsA_p5	AAATTGGTAGACACGCTGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	57.8	58.0	1755-1757 bp	1.0	79.1