Hotspot

rpl32-trnL-CUA

SSC · 111776-112766 · 991 bp

Back to Noccaea

Pi 0.0205

MarkerSeek score 67.50

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 991
variable_sites: 19
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	1070-1125 bp	1.0	70.2
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	1063-1118 bp	1.0	69.7
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	1069-1124 bp	1.0	69.6
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	1068-1123 bp	1.0	69.6
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	1064-1119 bp	1.0	69.0