Hotspot

rpl33-rps18

LSC · 65751-65958 · 208 bp

Back to Noccaea

Pi 0.0539

MarkerSeek score 83.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 11
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AATACCCCCAGTCGATTGGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	375-377 bp	1.0	80.3
rpl33-rps18_p2	ATACCCCCAGTCGATTGGAA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	374-376 bp	1.0	80.3
rpl33-rps18_p3	TACCCCCAGTCGATTGGAAT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	373-375 bp	1.0	80.3
rpl33-rps18_p4	ATCGGCATAATACCCCCAGT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.3	57.7	383-385 bp	1.0	79.0
rpl33-rps18_p5	CCCCAGTCGATTGGAATTGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.6	57.7	370-372 bp	1.0	77.9