Hotspot

trnM-AUG-atpE

LSC · 51447-51586 · 140 bp

Back to Noccaea

Pi 0.0432

MarkerSeek score 77.10

Primer available yes

Label trnM-AUG-atpE

Feature Metadata

label_name: trnM-AUG-atpE
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 6
pi: 0.0432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-AUG-atpE_p1	GGCAGGAGTCATTGGTTCAA	TACACGAGTCGAGGCTCTTA	50.0	50.0	58.1	57.6	205-206 bp	1.0	78.9
trnM-AUG-atpE_p2	TCGATGTGATAGGTACCCCC	TACACGAGTCGAGGCTCTTA	55.0	50.0	58.0	57.6	289-290 bp	1.0	78.8
trnM-AUG-atpE_p3	GGCAGGAGTCATTGGTTCAA	ACACGAGTCGAGGCTCTTAA	50.0	50.0	58.1	58.5	204-205 bp	1.0	78.3
trnM-AUG-atpE_p4	TCGATGTGATAGGTACCCCC	ACACGAGTCGAGGCTCTTAA	55.0	50.0	58.0	58.5	288-289 bp	1.0	78.1
trnM-AUG-atpE_p5	TCGATGTGATAGGTACCCCC	AGCTGAAGGCAAGAGACAAA	55.0	45.0	58.0	57.4	343-344 bp	1.0	76.4