Hotspot

psbG-ndhC

LSC · 49400-49458 · 59 bp

Back to Noccaea

Pi 0.0172

MarkerSeek score 64.00

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GGCTGGAAAGTCTTGACCAA	TTTATGCATGGCGAAAGGGA	50.0	45.0	58.0	57.9	196-197 bp	1.0	79.6
psbG-ndhC_p2	GGCTGGAAAGTCTTGACCAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.0	57.8	197-198 bp	1.0	79.0
psbG-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	278-279 bp	1.0	78.8
psbG-ndhC_p4	GGCTGGAAAGTCTTGACCAA	TGTACTGGGGGTATCTGCTT	50.0	50.0	58.0	57.7	261-262 bp	1.0	78.4
psbG-ndhC_p5	GGCTGGAAAGTCTTGACCAA	GAGTTTCGATGTACTGGGGG	50.0	55.0	58.0	57.7	270-271 bp	1.0	78.0