Hotspot

trnG-GGA-trnR-AGA

LSC · 9472-9639 · 168 bp

Back to Noccaea

Pi 0.0539
MarkerSeek score 69.00
Primer available yes
Label trnG-GGA-trnR-AGA

Feature Metadata

label_name
trnG-GGA-trnR-AGA
feature_type
igs
region
LSC
length_bp
168
variable_sites
9
pi
0.0539
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGA-trnR-AGA_p1 GGGTTCGATTCCCGCTAC TGATATTGCGTCCAATAGGA 61.1 40.0 57.6 53.9 269 bp 1.0 43.0
trnG-GGA-trnR-AGA_p2 GGTTCGATTCCCGCTACC TGATATTGCGTCCAATAGGA 61.1 40.0 57.6 53.9 268 bp 1.0 43.0
trnG-GGA-trnR-AGA_p3 GTTCGATTCCCGCTACCC TGATATTGCGTCCAATAGGA 61.1 40.0 57.6 53.9 267 bp 1.0 43.0
trnG-GGA-trnR-AGA_p4 GGTTCGATTCCCGCTACC TTGATATTGCGTCCAATAGGA 61.1 38.1 57.6 54.8 269 bp 1.0 43.0
trnG-GGA-trnR-AGA_p5 GTTCGATTCCCGCTACCC TTGATATTGCGTCCAATAGGA 61.1 38.1 57.6 54.8 268 bp 1.0 43.0