Hotspot

ycf1

SSC · 128725-134280 · 5556 bp

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Pi 0.00234

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5556
variable_sites: 31
pi: 0.00234
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGCA	42.9	30.8	58.0	57.0	227 bp	1.0	41.4
ycf1_p2	TGCTTGTGACTGTGAGAAACA	ACCCTTAATATTCCAAAAATCAATGC	42.9	30.8	58.0	57.0	228 bp	1.0	41.4
ycf1_p3	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGCAA	42.9	29.6	58.0	57.6	227 bp	1.0	41.4
ycf1_p4	TGCTTGTGACTGTGAGAAACA	ACCCTTAATATTCCAAAAATCAATGCA	42.9	29.6	58.0	58.5	228 bp	1.0	41.4
ycf1_p5	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGC	42.9	32.0	58.0	55.5	227 bp	1.0	41.4