Hotspot

rpl32-trnL-UAG

SSC · 118019-119057 · 1039 bp

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Pi 0.00193
MarkerSeek score 60.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1039
variable_sites
5
pi
0.00193
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCCTTTCTACCGGGAATTCG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1126-1133 bp 1.0 80.4
rpl32-trnL-UAG_p2 CGAAATCCCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1132-1139 bp 1.0 80.4
rpl32-trnL-UAG_p3 CCCTTTCTACCGGGAATTCG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1127-1134 bp 1.0 80.4
rpl32-trnL-UAG_p4 CGAAATCCCTTTCTACCGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1133-1140 bp 1.0 80.4
rpl32-trnL-UAG_p5 ACCGGGAATTCGAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1118-1125 bp 1.0 80.0