Hotspot

ndhF-rpl32

SSC · 116801-117844 · 1044 bp

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Pi 0.00479

MarkerSeek score 62.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1044
variable_sites: 11
pi: 0.00479
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTGGGACGAAAGGTATGACC	AAAAAGCTTTCAACGCTGCC	55.0	45.0	58.0	58.4	1183-1188 bp	1.0	77.8
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	1236-1241 bp	1.0	77.5
ndhF-rpl32_p3	ACGAAAGGTATGACCCAGGA	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.1	58.4	1178-1183 bp	1.0	77.5
ndhF-rpl32_p4	GTGGGACGAAAGGTATGACC	CGCTGCCCAATATCCCTTTT	55.0	50.0	58.0	58.6	1170-1175 bp	1.0	76.4
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	AAAAAGCTTTCAACGCTGCC	45.0	45.0	57.8	58.4	1238-1243 bp	1.0	76.3