Hotspot

ycf2-trnL-CAA

IRb · 95613-97880 · 2268 bp

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Pi 0.000178

MarkerSeek score 50.80

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 2268
variable_sites: 1
pi: 0.000178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	TTCTATAGGAACCGGACGCT	AAATGGTAGACACGCGAGAC	50.0	50.0	57.9	58.0	2479-2564 bp	1.0	82.2
ycf2-trnL-CAA_p2	TTCTATAGGAACCGGACGCT	TGAAATGGTAGACACGCGAG	50.0	50.0	57.9	58.0	2481-2566 bp	1.0	82.2
ycf2-trnL-CAA_p3	TTCTATAGGAACCGGACGCT	ATGTCCGATTGGGGCCTATA	50.0	50.0	57.9	58.0	2619-2704 bp	1.0	81.8
ycf2-trnL-CAA_p4	TTCTATAGGAACCGGACGCT	TGTCCGATTGGGGCCTATAT	50.0	50.0	57.9	58.0	2618-2703 bp	1.0	81.8
ycf2-trnL-CAA_p5	TTCTATAGGAACCGGACGCT	CCAACACTCCACCTTTGTCA	50.0	50.0	57.9	57.9	2573-2658 bp	1.0	81.7