Hotspot

petN-psbM

LSC · 31001-32041 · 1041 bp

Back to Nitraria

Pi 0.00212

MarkerSeek score 53.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 5
pi: 0.00212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	1194-1196 bp	1.0	64.7
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	1200-1202 bp	1.0	63.2
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1140-1142 bp	1.0	62.0
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	1194-1196 bp	1.0	60.4
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	50.0	45.0	57.8	55.5	1200-1202 bp	1.0	58.9