Hotspot

atpH-atpI

LSC · 15298-16459 · 1162 bp

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Pi 0.000689

MarkerSeek score 51.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 2
pi: 0.000689
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCGGCAGAAATCATTGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1239-1241 bp	1.0	78.7
atpH-atpI_p2	AAGCAAGTCCTACAGCCAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.1	57.9	1276-1278 bp	1.0	78.4
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	1245-1247 bp	1.0	78.4
atpH-atpI_p4	ACAGAAGCGGCAGAAATCAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1244-1246 bp	1.0	78.2
atpH-atpI_p5	AAGCGGCAGAAATCATTGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1240-1242 bp	1.0	77.9