Hotspot

matK-trnK-UUU

LSC · 3777-4495 · 719 bp

Back to Nitraria

Pi 0.00223

MarkerSeek score 60.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 4
pi: 0.00223
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AAGTTGCGTTGCTGAGATCT	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.8	58.2	796-798 bp	1.0	77.6
matK-trnK-UUU_p2	AAGTTGCGTTGCTGAGATCT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.8	58.2	795-797 bp	1.0	77.6
matK-trnK-UUU_p3	TAGGAAGTTGCGTTGCTGAG	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.2	58.2	800-802 bp	1.0	77.4
matK-trnK-UUU_p4	TAGGAAGTTGCGTTGCTGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	799-801 bp	1.0	77.4
matK-trnK-UUU_p5	AAGTTGCGTTGCTGAGATCT	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.8	57.5	809-811 bp	1.0	75.3