Hotspot

trnH-GUG-psbA

LSC · 93-634 · 542 bp

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Pi 0.00554

MarkerSeek score 50.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 7
pi: 0.00554
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTAGTTATGGGCGAACGACG	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.1	58.0	720-724 bp	1.0	79.8
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	658-662 bp	1.0	79.6
trnH-GUG-psbA_p3	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	673-677 bp	1.0	79.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	672-676 bp	1.0	79.5
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	671-675 bp	1.0	79.5