Hotspot

ycf1

SSC · 123790-128874 · 5085 bp

Back to Nigella

Pi 0.0386

MarkerSeek score 63.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5085
variable_sites: 623
pi: 0.0386
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACCTCCTTCCCCAACCAAT	CGGGACAATCTGAGCAATGA	50.0	50.0	57.9	58.0	849-870 bp	0.833333	82.5
ycf1_p2	CTGGTAATTCAGCACCGTCA	CGGGACAATCTGAGCAATGA	50.0	50.0	57.9	58.0	1156-1165 bp	0.777778	77.2
ycf1_p3	GTTGAAGCACTGACCCAGAA	CGGGACAATCTGAGCAATGA	50.0	50.0	58.0	58.0	745-751 bp	0.777778	76.6
ycf1_p4	GCACCGTCATAGTTGCAGTA	CGGGACAATCTGAGCAATGA	50.0	50.0	58.0	58.0	1145-1154 bp	0.666667	72.2
ycf1_p5	GGAATTCGTTTTCGGGCAAG	CGGGACAATCTGAGCAATGA	50.0	50.0	58.0	58.0	1340 bp	0.611111	64.4