Hotspot

ndhF-trnL-UAG

SSC · 113168-114038 · 871 bp

Back to Nigella

Pi 0.0269

MarkerSeek score 64.50

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 871
variable_sites: 93
pi: 0.0269
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	CAAAGGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	624-970 bp	1.0	91.6
ndhF-trnL-UAG_p2	CAAAGGGTATGATCCACGCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	625-971 bp	1.0	91.6
ndhF-trnL-UAG_p3	CAAAGGGTATGATCCACGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	634-980 bp	1.0	90.7
ndhF-trnL-UAG_p4	CAAAGGGTATGATCCACGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	633-979 bp	1.0	90.1
ndhF-trnL-UAG_p5	CAAAGGGTATGATCCACGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	632-978 bp	1.0	90.1