Hotspot

rpl22-rps19

LSC · 85320-85413 · 94 bp

Back to Nigella

Pi 0.0492

MarkerSeek score 61.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 94
variable_sites: 10
pi: 0.0492
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACTTCTACATCCGGGTCACT	GGACACGCGAGAAACGATAA	50.0	50.0	57.8	58.4	128-167 bp	1.0	85.1
rpl22-rps19_p2	ACTTCTACATCCGGGTCACT	GCACCTACTCGGAATTTCCA	50.0	50.0	57.8	57.6	149-188 bp	1.0	84.2
rpl22-rps19_p3	ACTTCTACATCCGGGTCACT	TGCACCTACTCGGAATTTCC	50.0	50.0	57.8	57.6	150-189 bp	1.0	84.2
rpl22-rps19_p4	ACTTCTACATCCGGGTCACT	CTCGGAATTTCCAGGGACAC	50.0	55.0	57.8	58.3	142-181 bp	0.944444	82.8
rpl22-rps19_p5	TCTACATCCGGGTCACTCTT	CTCGGAATTTCCAGGGACAC	50.0	55.0	57.5	58.3	139-178 bp	0.944444	80.5