Hotspot

trnT-UGU-trnF-GAA

LSC · 46964-48765 · 1802 bp

Back to Nigella

Pi 0.0175

MarkerSeek score 64.50

Primer available yes

Label trnT-UGU-trnF-GAA

Feature Metadata

label_name: trnT-UGU-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1802
variable_sites: 102
pi: 0.0175
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1813-1950 bp	1.0	86.8
trnT-UGU-trnF-GAA_p2	GCGATGCTCTAACCTCTGAG	TTAACCACATGCCAGGAACC	55.0	50.0	57.9	58.1	1836-1973 bp	1.0	86.6
trnT-UGU-trnF-GAA_p3	GCGATGCTCTAACCTCTGAG	CACATGCCAGGAACCAGATT	55.0	50.0	57.9	58.2	1831-1968 bp	1.0	86.0
trnT-UGU-trnF-GAA_p4	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	1845-1982 bp	1.0	85.9
trnT-UGU-trnF-GAA_p5	GGAATCGAACCGATGACCAT	TTAACCACATGCCAGGAACC	50.0	50.0	57.8	58.1	1868-2005 bp	1.0	85.6