Hotspot

trnY-GUA-trnE-UUC

LSC · 30966-31033 · 68 bp

Back to Nigella

Pi 0.0444

MarkerSeek score 62.40

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 8
pi: 0.0444
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	177-200 bp	1.0	81.8
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	178-201 bp	1.0	81.7
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	55.0	55.0	58.4	58.3	156-179 bp	1.0	81.1
trnY-GUA-trnE-UUC_p4	ACGAATTTACAGTCCGTCCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.6	58.3	157-180 bp	1.0	80.9
trnY-GUA-trnE-UUC_p5	GCCAACGAATTTACAGTCCG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.5	58.3	161-184 bp	1.0	79.6