Hotspot

trnD-GUC-trnY-GUA

LSC · 30492-30881 · 390 bp

Back to Nigella

Pi 0.0313

MarkerSeek score 62.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 34
pi: 0.0313
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATACTAGGTCGGGACTGACG	TGGCAATATGTCTACGCTGG	55.0	50.0	58.0	57.8	502-513 bp	1.0	85.3
trnD-GUC-trnY-GUA_p2	ATACTAGGTCGGGACTGACG	GGCAATATGTCTACGCTGGT	55.0	50.0	58.0	57.8	501-512 bp	1.0	85.3
trnD-GUC-trnY-GUA_p3	ATACTAGGTCGGGACTGACG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.0	58.4	523-534 bp	1.0	84.1
trnD-GUC-trnY-GUA_p4	ATACTAGGTCGGGACTGACG	GGGACGGACTGTAAATTCGT	55.0	50.0	58.0	57.6	522-533 bp	1.0	84.0
trnD-GUC-trnY-GUA_p5	CATCGGATACTAGGTCGGGA	TGGCAATATGTCTACGCTGG	55.0	50.0	57.8	57.8	508-519 bp	1.0	84.0