Hotspot

petN-psbM

LSC · 28052-29232 · 1181 bp

Back to Nigella

Pi 0.0151

MarkerSeek score 66.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 64
pi: 0.0151
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1285-1336 bp	1.0	76.6
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1291-1342 bp	1.0	75.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1231-1282 bp	1.0	74.0
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1285-1336 bp	1.0	72.4
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	57.8	56.1	1291-1342 bp	1.0	71.0