Hotspot

rpl32-trnL-UAG

SSC · 115124-116060 · 937 bp

Back to Nicotiana

Pi 0.018

MarkerSeek score 64.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 937
variable_sites: 71
pi: 0.018
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTGTACGCCAAACAAAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	52.8	57.9	893-1006 bp	1.0	49.9
rpl32-trnL-UAG_p2	TTTGTACGCCAAACAAAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	52.8	57.8	886-999 bp	1.0	49.9
rpl32-trnL-UAG_p3	TTTGTACGCCAAACAAAAAT	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	52.8	57.8	892-1005 bp	1.0	49.9
rpl32-trnL-UAG_p4	TTTGTACGCCAAACAAAAAT	CTAAGAGCAGCGTGTCTACC	30.0	55.0	52.8	57.8	891-1004 bp	1.0	49.9
rpl32-trnL-UAG_p5	TTTGTACGCCAAACAAAAAT	TCGAACCGAGATGCATTAGC	30.0	50.0	52.8	58.1	919-1032 bp	1.0	49.7