Hotspot

ndhF-rpl32

SSC · 114169-114955 · 787 bp

Back to Nicotiana

Pi 0.0141
MarkerSeek score 62.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
787
variable_sites
54
pi
0.0141
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGAAAGGTATGATCCACGCA TGATGTCGAAGTGCGTTTTT 45.0 40.0 56.9 56.6 836-860 bp 1.0 68.2
ndhF-rpl32_p2 TGAAAGGTATGATCCACGCA TTGATGTCGAAGTGCGTTTT 45.0 40.0 56.9 56.6 837-861 bp 1.0 68.2
ndhF-rpl32_p3 TGAAAGGTATGATCCACGCA TTTGATGTCGAAGTGCGTTT 45.0 40.0 56.9 56.6 838-862 bp 1.0 68.2
ndhF-rpl32_p4 TGAAAGGTATGATCCACGCA TTTTGATGTCGAAGTGCGTT 45.0 40.0 56.9 56.6 839-863 bp 1.0 68.2
ndhF-rpl32_p5 TGAAAGGTATGATCCACGCA TTTTTGATGTCGAAGTGCGT 45.0 40.0 56.9 56.6 840-864 bp 1.0 68.2