Hotspot

ycf1-ndhF

SSC · 111910-111939 · 30 bp

Back to Nicotiana

Pi 0.034

MarkerSeek score 58.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 30
variable_sites: 5
pi: 0.034
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	GCGGACGCATCTCTTCTTAT	50.0	50.0	58.0	57.9	300-305 bp	1.0	86.7
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	TCAACCAAGAGGGAGGGAAT	50.0	50.0	58.0	57.9	747-752 bp	0.933333	80.8
ycf1-ndhF_p3	AGGGTGGAAAGTGAGGAAGA	TCAACCAAGAGGGAGGGAAT	50.0	50.0	57.9	57.9	812-817 bp	0.933333	79.9
ycf1-ndhF_p4	ACAGGAACAAGAGGGATCCA	TTCAACCAAGAGGGAGGGAA	50.0	50.0	58.0	58.2	748-753 bp	0.933333	79.9
ycf1-ndhF_p5	ACAGGAACAAGAGGGATCCA	CCAAGAGGGAGGGAATTTGG	50.0	55.0	58.0	57.9	743-748 bp	0.866667	76.4