Hotspot

rps19-rpl2

IRb · 86532-86593 · 62 bp

Back to Nicotiana

Pi 0.0175

MarkerSeek score 60.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 62
variable_sites: 5
pi: 0.0175
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	197-218 bp	1.0	77.0
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	59.2	58.3	199-220 bp	1.0	75.9
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	264-285 bp	1.0	75.4
rps19-rpl2_p4	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.6	58.1	196-217 bp	1.0	75.3
rps19-rpl2_p5	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	255-276 bp	1.0	75.1