Hotspot

psbM-trnD-GUC

LSC · 30907-31980 · 1074 bp

Back to Nicotiana

Pi 0.00749

MarkerSeek score 65.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 37
pi: 0.00749
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.4	58.0	1215-1247 bp	1.0	64.2
psbM-trnD-GUC_p2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	42.9	50.0	56.4	58.1	1216-1248 bp	1.0	63.6
psbM-trnD-GUC_p3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	40.9	55.0	57.1	58.0	1216-1248 bp	1.0	62.0
psbM-trnD-GUC_p4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.1	58.0	1212-1244 bp	1.0	62.0
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	40.9	50.0	57.1	58.1	1217-1249 bp	1.0	61.4