Hotspot

atpH-atpI

LSC · 14112-15276 · 1165 bp

Back to Nicotiana

Pi 0.00903

MarkerSeek score 58.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 48
pi: 0.00903
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.0	58.0	1292-1311 bp	1.0	83.8
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.2	58.0	1293-1312 bp	1.0	82.6
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1307-1326 bp	1.0	82.3
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TACCTGTCATGCTCCTTGGA	50.0	50.0	58.3	58.0	1302-1321 bp	1.0	82.0
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	57.6	58.0	1303-1322 bp	1.0	80.7