Hotspot

rps16-trnQ-UUG

LSC · 6204-7410 · 1207 bp

Back to Nicotiana

Pi 0.0268
MarkerSeek score 76.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1207
variable_sites
147
pi
0.0268
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1210-1308 bp 1.0 90.3
rps16-trnQ-UUG_p2 CACAACGGATCCTGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1230-1321 bp 0.933333 86.5
rps16-trnQ-UUG_p3 CGGATCCTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.7 58.1 1225-1316 bp 0.933333 86.3
rps16-trnQ-UUG_p4 GGATCCTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.7 58.1 1224-1315 bp 0.933333 86.3
rps16-trnQ-UUG_p5 GATCCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1223-1314 bp 0.933333 85.9