Hotspot

trnK-UUU-rps16

LSC · 4354-5069 · 716 bp

Back to Nicotiana

Pi 0.018
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
716
variable_sites
42
pi
0.018
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCGTCCTAATCAATCC 50.0 43.5 58.2 58.0 728-776 bp 1.0 62.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGAACTTCGTCCTAATCAATCCT 50.0 43.5 58.2 58.0 727-775 bp 1.0 62.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AGGAACTTCGTCCTAATCAATCC 50.0 43.5 58.2 58.0 727-775 bp 1.0 62.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGAACTTCGTCCTAATCAATCCT 50.0 43.5 58.2 58.0 726-774 bp 1.0 62.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGAACTTCGTCCTAATCAATCCT 50.0 43.5 58.3 58.0 736-784 bp 1.0 61.9