Hotspot

rpl32-trnL-UAG

SSC · 117490-118917 · 1428 bp

Back to Neviusia

Pi 0.00939
MarkerSeek score 67.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1428
variable_sites
13
pi
0.00939
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTTTCTACCGGGAGTTCGA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 1472-1506 bp 1.0 81.4
rpl32-trnL-UAG_p2 TCTTTCTACCGGGAGTTCGA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 1473-1507 bp 1.0 81.3
rpl32-trnL-UAG_p3 ATCGGGCAGCATTAAAAGCT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1509-1543 bp 1.0 81.1
rpl32-trnL-UAG_p4 ATCGGGCAGCATTAAAAGCT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1510-1544 bp 1.0 81.1
rpl32-trnL-UAG_p5 TCTTTCTACCGGGAGTTCGA TTAGAAGATGTCATGCCGCC 50.0 50.0 57.8 58.0 1536-1570 bp 1.0 80.9