Hotspot

ndhF-rpl32

SSC · 116260-117288 · 1029 bp

Back to Neviusia

Pi 0.00584
MarkerSeek score 62.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1029
variable_sites
6
pi
0.00584
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCACATACGACGAAGGTTTT GGTTCTTCGGTTGGATGTGA 45.0 50.0 56.1 57.8 1176-1177 bp 1.0 63.8
ndhF-rpl32_p2 CCACATACGACGAAGGTTTT AGGTTCTTCGGTTGGATGTG 45.0 50.0 56.1 57.8 1177-1178 bp 1.0 63.8
ndhF-rpl32_p3 CCACATACGACGAAGGTTTTT GGTTCTTCGGTTGGATGTGA 42.9 50.0 56.8 57.8 1176-1177 bp 1.0 61.8
ndhF-rpl32_p4 CCACATACGACGAAGGTTTTT AGGTTCTTCGGTTGGATGTG 42.9 50.0 56.8 57.8 1177-1178 bp 1.0 61.8
ndhF-rpl32_p5 AAGTAGAAGTCCTGCTCCAA GGTTCTTCGGTTGGATGTGA 45.0 50.0 55.8 57.8 1140-1141 bp 1.0 61.4