Hotspot

rps19-rpl2

IRb · 87719-87788 · 70 bp

Back to Neviusia

Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 2
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CGCTCGGGACCAAGTTACTA	GGGCCCCAATTGGTAGAAAA	55.0	50.0	59.2	58.1	300 bp	1.0	70.6
rps19-rpl2_p2	CGCTCGGGACCAAGTTACTA	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	208 bp	1.0	70.5
rps19-rpl2_p3	GCTCGGGACCAAGTTACTAT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	56.7	58.1	299 bp	1.0	69.9
rps19-rpl2_p4	GCTCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.7	58.1	207 bp	1.0	69.8
rps19-rpl2_p5	CGCTCGGGACCAAGTTACTA	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	266 bp	1.0	69.4