Hotspot

ndhC-trnV-UAC

LSC · 53923-54565 · 643 bp

Back to Neviusia

Pi 0.0202

MarkerSeek score 70.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 13
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	838-880 bp	1.0	78.9
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	774-816 bp	1.0	78.7
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	841-883 bp	1.0	77.7
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	840-882 bp	1.0	77.7
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	855-897 bp	1.0	77.3