Hotspot

trnL-UAA-trnF-GAA

LSC · 51026-51446 · 421 bp

Back to Neviusia

Pi 0.0166

MarkerSeek score 60.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 7
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	475 bp	1.0	77.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	511 bp	1.0	76.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	526 bp	1.0	76.5
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	487 bp	1.0	76.3
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTATTCGTGTGCCAGGAACC	50.0	50.0	57.5	58.2	534 bp	1.0	75.8