Hotspot

psaA-ycf3

LSC · 44143-44889 · 747 bp

Back to Neviusia

Pi 0.00402

MarkerSeek score 61.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 3
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	925-969 bp	1.0	79.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	927-971 bp	1.0	78.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	921-965 bp	1.0	77.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	923-967 bp	1.0	77.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	899-943 bp	1.0	76.5