Hotspot

trnT-GGU-psbD

LSC · 33332-34888 · 1557 bp

Back to Neviusia

Pi 0.00271
MarkerSeek score 65.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1557
variable_sites
4
pi
0.00271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1667-1721 bp 1.0 83.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1670-1724 bp 1.0 83.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1669-1723 bp 1.0 83.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GAAGAGCAATAGGCCAGACC 55.0 55.0 58.1 58.0 1678-1732 bp 1.0 83.1
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GGAAGAGCAATAGGCCAGAC 55.0 55.0 58.1 58.0 1679-1733 bp 1.0 83.1