Hotspot

clpP

LSC · 74083-76872 · 2790 bp

Back to Neuwiedia

Pi 0.0189

MarkerSeek score 64.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2790
variable_sites: 37
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGATCTTAACCTGCTGATGGT	AAAACGATGAAGTGAAGACA	47.6	35.0	57.8	53.1	2155-2991 bp	1.0	45.2
clpP_p2	CGATCTTAACCTGCTGATGGT	AAACGATGAAGTGAAGACAA	47.6	35.0	57.8	53.1	2154-2990 bp	1.0	45.2
clpP_p3	CGATCTTAACCTGCTGATGGT	AAAACGATGAAGTGAAGACAA	47.6	33.3	57.8	54.0	2155-2991 bp	1.0	45.2
clpP_p4	CGATCTTAACCTGCTGATGGT	AACGATGAAGTGAAGACAAT	47.6	35.0	57.8	52.8	2153-2989 bp	1.0	45.2
clpP_p5	CGATCTTAACCTGCTGATGGT	AAAAACGATGAAGTGAAGACA	47.6	33.3	57.8	54.0	2156-2992 bp	1.0	45.1